2011考研細胞生物學專業測試題附答案

2011考研細胞生物學專業測試題附答案

一、填空題

1 . Cyclic AMP is produced by the enzyme________and degraded by the enzyme________

2 . NO是一種氣體信號分子,在體內主要由精氨酸產生, 它的半衰期很短, 故只作用于鄰近細胞, 它作用的靶酶是________

3. ________junctions enable cells to get a hold on the extracellular matrix by connecting their actin filaments to the matrix.

4  動物細胞質膜靠________建立膜兩側的質子動力勢，而植物細胞則是靠________。

5. 蛋白質合成時多聚核糖體的形成對生命活動的意義在于：________。

二、判斷題

1. Active transport is always driven by the hydrolysis of high-energy phosphate bonds.(     )

2. Treatment of an animal cell with an inhibitor that is specific for the Na+/K+ pump is not likely to affect the uptake of glucose by sodium cotransport.(     )

3. Tight junctions get their name from their property of holding cells togetherso tightly that they cannot be separated by mechanical forces.（      ）

4. Gap junctions connect the cytoskeletal elements of one cell to a neighboring cell or to the extracellular matrix.（      ）

5. NO acts only locally because it has a short half-life--about 5 to 10 seconds--in the extracellular space before it is converted to nitrates and nitrites by oxygen and water.(     )

三、選擇題

1.  Which one of the following was used to determine the structure of the DNA molecule?(      )

a. Transmission electron microscope    b. Scanning electron microscope

c. Differential centrifugatioin         d. X-ray crystallography

e. None of the above

2.  You stain membrane proteins with a fluorescent-labeled antibody and notice the membrane is evenly stained. After a few hours, all of the fluorescence is at one end of a cell. You can conclude that(     ).

a. Lipids flip-flop in the membrane.   b. Proteins move laterally in the membrane.

c. The fluorescent dye was bleached.  d. Proteins act as transporters.

e. All of the above

3.  You have a preparation of ghosts and need to collect only the right-side out ghosts. You decide you can trap the right-side out ghosts in an affinity column packed with(     )

a. SDS.                        b. A carbohydrate-binding molecule.

c. A protein-binding antibody.      d. A serine-binding molecule.

e. None of the above

4.  All of the following individuals contributed to cell theory except(      )

a.  Robert Hooke.            b. Matthias Schleiden.

c.  Theodor Schwann.        d.  Rudolf Virchow.

5.  The addition of a peptide with an RGD sequence would probably  ?  the binding of cultured cells to a fibronectin coated dish.(     )

a.  enhance              b. inhibit

c.  have no effect on      d.   none of the above